Bioassay guided fractionation pdf !237!

Bio-Assay Guided Fractionation and Isolation of ?-Glucosidase Inhibitory Constituents from .. DOI: 10.9790/3008-1206026874 www.iosrjournals.org 69 | Page 2.1 Selection of plant Based on the traditional uses and reported scientific contributions the plant D. indica was selected for the

Bioassay-guided fractionation of the A. belladonna extract resulted in the identification of lycorine as the bio-active compound. The IC 50 measured for radicle growth inhibition was 0.10 µM for narciclasine and 0.93 µM for lycorine, compared to 0.11 mM of chlorpropham, a synthetic herbicide. A central challenge of natural products research is assigning bioactive compounds from complex mixtures. The gold standard approach to address this challenge, bioassay-guided fractionation, is often biased toward abundant, rather than bioactive, mixture components. This study evaluated the combination of bioassay-guided fractionation with untargeted metabolite profiling to improve active 6. . BIOASSAY GUIDED FRACTIONATION AND STRUCTURAL ELUCIDATION OF BIOACTIVE METABOLITE FROM THE BRYOZOANN Zoobotryon verticillatum INTRODUCTION Marine organisms have formed a valuable source of new bioactive compounds, many of which are being used in the treatment of many diseases Methods: Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumis prophetarum var. prophetarum fruits. These compounds were structurally elucidated using NMR spectroscopy, mass spectral analyses and x-ray crystallography. All fractions, sub-fractions and pure compounds were screened for their PDF | It is a common problem in natural product therapeutic lead discovery programs that despite good bioassay results in the initial extract, the active compound(s) may not be isolated during the bioassay guided fractionation and isolation of Lupenol and its ?-amylase inhibitory activity for the first time for this plant. II. Materials and Methods Plant collection: The stem bark of F